Advantages of fluorescence in situ hybridisation technique in urothelial carcinogenesis identification. Retrospective evaluation of 512 UroVysion test results

Abstract: There is a time point with cellular genome destabilisation, observed in the course of carcinogenesis process. Aneuploidies 3, 7 and 17, as well as the loss of 9p21 are among the most frequent events, observed in the course of genome destabilisation (included in the UroVysion Bladder Cancer Kit). During 5 years, we carried out 512 UroVysion tests; 68% of examined patients were referred for diagnostics of urinary bladder cancer, 8.4% for therapeutic effect follow up, 2% for dysuria, 2.7% for haematuria, 0.5% for chronic, recurrent cystitis, 1.4% for other indications and 13.1% for no precise indications. Twenty-five (25) morphologically abnormal cells were evaluated. The following diagnostic criteria were established: a result was regarded positive when more than 10 cells demonstrated 9p21 fragment loss (the presence of one or the loss of both signals) or if more than 4 cells showed polysomy of two chromosomes (among chromosomes 3, 7, 17) or if more than 10 cells indicated polysomy for one of the above-mentioned chromosomes. Positive results were obtained in 56% of the cases. In the group with negative results, our attention was drawn to a statistically higher percent of rearrangements in 9p21 region, compared to changes in chromosomes 3, 7, 17. That observation did confirm the assumption that the change in question is the earliest one in the process of carcinogenesis. It was surprising though that the change was identified in the group of patients with negative test results.

Tadeusz Kaluzewski, Lucyna Morzuch, Adam Jedrzejczyk, Piotr Marks, Marek Rozniecki, Michal Bednarek

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