Vital staining with Lugol's solution is performed at the time of upper endoscopy to aid in cancer detection. Lugol's staining involves the application of a solution that contains potassium iodide and iodine through a spray catheter. The dye stains the glycogen in normal squamous epithelium a dark brown color. Areas that are unstained, particularly those that are larger than 5 mm in size, are likely to be dysplasic or malignant and can be readily targeted for endoscopic biopsy. Smaller unstained areas (less than 5 mm) may result from inflammatory change.
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