MEDtube Science 2015 - Fragments od bacterial DNA presence in hemodialisis patients’ blood-preliminary report

Cardiovascular disease (CVD) accounts for most of morbidity and mortality in patients with end-stage renal disease treated by chronic hemodialysis. The idea that inflammation state present in uremia plays an important role in the development of atherosclerosis has enjoyed much attention. We have investigated therefore pos- sible presence of bacterial DNA in the blood of chronically hemodialyzed patients. There were retrospectively studies of 59 patients without any signs of active infection, who had been undergoing intermittent HD. In 54 among 59 investigated DNA blood samples from HD patients we were able to detect a DNA fragment about 790 bp in length, consistent with the presence of 16sRNA bacterial DNA. Bacterial DNA could not be detected in any of the samples from control subjects. We were not able to detect bacterial DNA in any of 9 investigated dialysate samples although in 4 blood samples of these patients bacterial DNA encoding ribosomal RNA was observed. Bacterial DNA is detectable in blood of HD patients. This might be one of the inflammatory stimuli.