Stroke and ibuprofen – brain tissue regeneration?

Scientists from a team of professor Małgorzata Kossut from Experimental Biology Institute of M. Nencki in Warsaw announced that using popular anti-inflammatory drugs may facilitate brain tissue regeneration after cerebral crisis in rats.

The aim of the researchers was to prove that popular anti-inflammatory drugs like ibuprofen may induce and accelerate reconstructive processes in rats’ cortical cortex after ischemic stroke.

A group of several dozens of rats was used for this aim. In the first phase of the experiment the researchers examined i.a. neuroplastic properties of the brain tissue in the animals. In order to do that they were cutting off single whiskers on the rats’ heads and observed how other hairs took representations of this area. Because of stimulation of brain areas adjacent with denervated ones by touching the rest of whiskers the process was quick. In this way it was proved that rats’ brain tissue has neuroplastic properties.

The next step was to cause ischemic stroke in animals’ brain tissue in the area representing whiskers. It turned out that in this case there is no taking up of functions by adjacent nerve centres – regeneration is impaired. Inflammation was diagnosed in the area of the focal point of ischaemia what is connected with escalation of Cyclooxygenase-2 (COX-2) level in cortex. The level of COX-2 was tested with the use of Western blot method. The scientists assumed that inflammation prevention may improve neuroplastic properties in rats’ brain tissue.


After causing the stroke ibuprofen was administered in a dose of 10mg/kg of body weight and 20mg/kg of body weight. The animals were given the drug one day before the stroke and every day after it for a month. A condition of rats’ cortex was examined. The results were compared with control groups.

It was proved that after administration of ibuprofen to the animals, along with the inflammation withdrawal the ability of brain tissue to regroup reappear. What is more, the effect escalates with the dosage increase. Ibuprofen administered before the stroke does not influence the neuroplasticity of healthy brain.

Research done on rats and the conclusions presented in numerous thesis allow to claim that the phenomena of neuroplasticity increase after using anti-inflammatory drugs may take place also in humans with ischaemic changes of the brain. It is worth to delve into the question in order to affirm this thesis and maybe in the future – to add ibuprofen to the basic treatment algorism of the patients with fresh ischemic stroke of the brain.

Written by: Julia Rudnicka, Anna Kozioł, Jerzy Bednarski

Source:
1.Jablonka JA, et. al. Experience-dependent brain plasticity after stroke: effect of ibuprofen and poststroke delay. Eur J Neurosci. 2012 Sep;36(5):2632-9. doi: 10.1111/j.1460-9568.2012.08174.x. Epub 2012 Jun 13.
2.Bazan NG et.al. Novel aspirin-triggered neuroprotectin D1 attenuates cerebral ischemic injury after experimental stroke. Exp Neurol. 2012 Jul;236(1):122-30. doi: 10.1016/j.expneurol.2012.04.007.
3.Wang J. et.al. Anti-inflammation and antioxidant effect of Cordymin, a peptide purified from the medicinal mushroom Cordyceps sinensis, in middle cerebral artery occlusion-induced focal cerebral ischemia in rats. Metab Brain Dis. 2012 Jun;27(2):159-65. doi: 10.1007/s11011-012-9282-1.
4.Park JS et.al. Anti-inflammatory mechanism of compound K in activated microglia and its neuroprotective effect on experimental stroke in mice. J Pharmacol Exp Ther. 2012 Apr;341(1):59-67. doi: 10.1124/jpet.111.189035.
5.Liebigt S. et.al. Effects of rehabilitative training and anti-inflammatory treatment on functional recovery and cellular reorganization following stroke. Exp Neurol. 2012 Feb;233(2):776-82. doi: 10.1016/j.expneurol.2011.11.037.
6. Wang W. et.al. High doses of salicylate and aspirin are inhibitory on acid-sensing ion channels and protective against acidosis-induced neuronal injury in the rat cortical neuron. J Neurosci Res. 2012 Jan;90(1):267-77. doi: 10.1002/jnr.22742.

Would you like to know more? Watch on MEDtube.net: Stroke Evaluation Simulation 1

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